Influenza virus uncoating in infected cells and effect of rimantadine.

Inhibition of influenza virus uncoating by rimantadine hydrochloride.

In freeze-thaw lysates of MDCK cells infected with 32P-labeled influenza virus A/WSN in the presence of added RNase, acid-precipitable radioactivity diminished to about 50% of initial values within 90 min after a 1-h virus adsorption period. A similar preparation containing rimantadine at a concentration of 50 micrograms/ml exhibited only a 10% reduction in acid-precipitable radioactivity. Thes...

Different modes of inhibition by adamantane amine derivatives and natural polyamines of the functionally reconstituted influenza virus M2 proton channel protein.

The influenza virus M2 protein, target of the antiviral drugs amantadine and rimantadine, forms a proton channel which functions during virus uncoating and maturation by modifying the pH in virions as well as in trans-Golgi vesicles. We studied the influence of different ionic gradients on the inhibition of the proton translocation activity of isolated, baculovirus-expressed M2 protein reconsti...

The Potential Effect of Glycyrrhiza Glabra on Early Step of Influenza Virus Replication

Background and Aims: The emergence of drug-resistant influenza viruses has become a serious threat for human and animal populations. Glycyrrhiza glabra (Gg) is a traditional medicine clinically used for the treatment of viral respiratory infection symptoms in most countries. We evaluated the effects of the herb on influenza virus replication in human lung cultured cells (A549) following the det...

Effect of Lysozyme in maintenance Medium for FL cells infected with Para-Influenza 3 virus

Interfering With Lipid Raft Association: A Mechanism to Control Influenza Virus Infection By Sambucus Nigra

Sambucus nigra (elder) are broadly used species to treat microbial infections. Thepotential antiviral activity and mechanism action of elder fruit (EF) in human epithelium cell(A549) cultures infected with H9N2 influenza virus were determined. The effect of variousconcentrations of EF on influenza virus replication was examined by using virus titration,quantitative real time RT-PCR, fusion and ...